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KMID : 0545120070170040650
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 4 p.650 ~ p.654
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Optimization of Lipase Pretreatment Prior to Lipase Immobilization to Prevent Loss of Activity
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Lee Dong-Hwan
Kim Jung-Mo
Shin Hyun-Yong
Kim Seung-Wook
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Abstract
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In our previous work, a method of pretreating lipase was developed to prevent loss of its activity during covalent immobilization. In this study, Rhizopus oryzae lipase was pretreated before immobilization and then immobilized on a silica gel surface. The effects of the various materials and conditions used in the pretreatment stage on the activity of immobilized lipase were investigated. Immobilized lipase pretreated with 0.1% of soybean oil had better activity than those pretreated with other materials. The optimal temperature, agitation speed, and pretreating time for lipase pretreatment were determined to be 40oC, 200 rpm, and 45 min, respectively. The activity of immobilized soybean oil pretreated lipase was 630 U/g matrix, which is 20 times higher than that of immobilized non-pretreated lipase. In addition, immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.
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KEYWORD
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Pretreatment, immobilization, lipase, soybean oil
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